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Updated: May 29, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Published on: September 28, 2018
Detection and characterization of receptor interactions with PDZ domains
Stefanie L Ritter1, Randy A Hall
1Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA.
Abstract:
Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.
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