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Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: May 29, 2026

Cell Cycle-specific Measurement of &#947;H2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

Analyzing p53 regulated DNA damage checkpoints by flow cytometry.

Lois Resnick-Silverman1, James J Manfredi

  • 1Department of Oncological Sciences, Mount Sinai School of Medicine, New York, NY 10018, USA. lois.resnick-silverman@mssm.edu

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2011
PubMed
Summary
This summary is machine-generated.

Cell cycle checkpoints pause cell division to repair DNA damage, preventing mutations. Flow cytometry analyzes DNA content to observe these cell cycle changes after damage.

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Area of Science:

  • Cell Biology
  • Genetics
  • Biotechnology

Background:

  • Cellular response to DNA damage is critical for preventing mutations.
  • Cell cycle checkpoints in mammalian cells halt progression to allow for DNA repair.
  • Cells arrest at specific DNA content levels (2N or 4N) during the cell cycle.

Purpose of the Study:

  • To explain the role of cell cycle checkpoints in DNA damage response.
  • To describe how flow cytometry is utilized to analyze cell cycle progression.
  • To demonstrate the application of flow cytometry in observing cell cycle alterations post-DNA damage.

Main Methods:

  • Flow cytometry, a technique utilizing light scattering and fluorescence, is employed.
  • Cells are fixed and permeabilized to enable DNA staining with fluorescent dyes.
  • Separation of cells based on DNA content (2N vs. 4N) is achieved.

Main Results:

  • Flow cytometry effectively sorts cells based on their DNA content.
  • Changes in the distribution of G1, S, and G2/M phases are observable.
  • The technique allows for the visualization of cell cycle arrest following DNA damage.

Conclusions:

  • Flow cytometry is a valuable tool for studying the cell cycle response to DNA damage.
  • Understanding cell cycle regulation is crucial for preventing genetic instability.
  • This method facilitates the analysis of DNA repair mechanisms and their impact on cell division.