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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Updated: May 29, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Polymeric sequence probe for single DNA detection.

Suxian Huang, Yong Chen

    Analytical Chemistry
    |August 30, 2011
    PubMed
    Summary
    This summary is machine-generated.

    A novel polymeric sequence probe (PSP) enables ultrasensitive detection of single molecules. This DNA probe amplifies fluorescence signals for clear visualization, demonstrated with a Mycobacterium tuberculosis assay.

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    DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
    08:04

    DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

    Published on: October 8, 2019

    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Biophysics

    Background:

    • Single molecule detection is crucial for understanding biological processes at the molecular level.
    • Existing methods for single molecule detection can be limited by signal strength and sensitivity.
    • Developing novel probes is essential for advancing ultrasensitive diagnostic tools.

    Discussion:

    • The polymeric sequence probe (PSP) utilizes tandem repeats of target-binding and label-binding sequences.
    • Multiple fluorescent oligos bind to a single PSP, significantly amplifying the signal.
    • This amplification allows for clear visualization of single target molecules using standard fluorescence microscopy.

    Key Insights:

    • PSP enables single-molecule detection with enhanced sensitivity.
    • Ultrasensitive detection of Mycobacterium tuberculosis was achieved using the PSP assay.
    • The probe design overcomes limitations of low target molecule concentrations.

    Outlook:

    • PSP technology holds potential for developing new ultrasensitive diagnostic tools.
    • Further applications in detecting other pathogens or biomarkers are feasible.
    • Optimization of PSP design could lead to even greater sensitivity and broader utility.