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Effects of the Hb E, Hb H and Hb G-Taichung variants on HbA1c values by the Bio-Rad variant II turbo analyzer
Su-Chen Lee1, Li-Hsuan Wang, Shih-Meng Tsai
1Department of Laboratory Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Objectives:
The influences of Hb variants on HbA(1c) values can cause mismanagement of diabetes; therefore, the effects of Hb E, H, and G-Taichung variants were evaluated.
Designs And Methods:
HbA(1c) values of 2105 samples, including 37 samples with Hb E, H and G-Taichung variants identified by Hb electrophoresis and the PCR sequence, were evaluated by the ion-exchange (Bio-Rad Variant II Turbo analyzer) and boronate affinity (Primus CLC 385 analyzer) high performance liquid chromatography (HPLC) methods.
Results:
In the patients with the Hb E and H variants, their HbA(1c) values determined by ion-exchange HPLC were significantly higher than those by boronate affinity HPLC. However, there were no significant differences of the HbA(1c) values in the patients with the Hb G-Taichung variant.
Conclusions:
The HbA1c levels might be interfered by the Hb E and H variants, but not the Hb G-Taichung variant, measured by the Bio-Rad Variant II Turbo analyzer.
