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Updated: May 29, 2026
13:08
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Structural basis of coagulation factor V recognition for cleavage by RVV-V
Daisuke Nakayama1, Youssef Ben Ammar, Toshiyuki Miyata
1Department of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan.
FEBS Letters
|August 30, 2011
Summary
Russell's viper venom factor V activator (RVV-V) precisely targets a specific bond in factor V. Its crystal structure reveals an induced-fit mechanism explaining RVV-V's high substrate specificity.
Area of Science:
- Biochemistry
- Structural Biology
- Enzymology
Background:
- Russell's viper venom factor V activator (RVV-V) is a serine protease.
- RVV-V exhibits high specificity, cleaving only the Arg1545-Ser1546 bond in factor V (FV).
- Understanding RVV-V's mechanism is crucial for its potential applications.
Purpose of the Study:
- To elucidate the structural basis of RVV-V's substrate specificity.
- To investigate the molecular interactions between RVV-V and its substrate.
Main Methods:
- Determined the crystal structure of RVV-V in complex with the FV14 peptide.
- Utilized X-ray crystallography at 1.8Å resolution.
- Compared substrate-bound and substrate-free RVV-V structures.
Main Results:
- Revealed multiple specific interactions between RVV-V and seven residues (Ile1539-Arg1545) of the FV14 peptide.
- Observed conformational changes in RVV-V loops upon substrate binding.
- Indicated an induced-fit mechanism governing substrate recognition and binding.
Conclusions:
- The study provides a detailed structural explanation for RVV-V's narrow substrate specificity.
- The induced-fit mechanism highlights the dynamic nature of enzyme-substrate interactions.
- Findings contribute to the understanding of protease specificity and function.
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