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Related Experiment Video

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Flow injection fluorometric determination of ascorbic acid using perylenebisimide-linked nitroxide.

Tomoharu Maki1, Nobuaki Soh, Koji Nakano

  • 1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.

Talanta
|August 30, 2011
PubMed
Summary
This summary is machine-generated.

A new flow injection method uses a fluorescent reagent, perylenebisimide-linked nitroxide (PBILN), for sensitive ascorbic acid detection. This technique recovers fluorescence, enabling accurate quantification in beverages.

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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Ascorbic acid (vitamin C) is vital for human health and widely present in food and beverages.
  • Accurate and sensitive quantification of ascorbic acid is crucial for quality control and nutritional analysis.
  • Existing methods for ascorbic acid determination may lack sensitivity or selectivity.

Purpose of the Study:

  • To develop a simple, sensitive, and selective flow injection fluorometric method for ascorbic acid determination.
  • To utilize the unique fluorescence quenching and recovery properties of perylenebisimide-linked nitroxide (PBILN).
  • To validate the method for analyzing ascorbic acid in real-world samples like soft drinks.

Main Methods:

  • A flow injection analysis (FIA) system was employed.
  • Perylenebisimide-linked nitroxide (PBILN) was synthesized and used as a fluorescent probe.
  • The reaction mechanism involves the quenching of PBILN fluorescence by its nitroxide moiety, which is reversed upon reaction with ascorbic acid.
  • Fluorescence detection was performed downstream of the reaction zone.

Main Results:

  • The method demonstrated a good linear relationship between ascorbic acid concentration and fluorescence signal peak height in the range of 0.5–10 μmol L⁻¹.
  • A low detection limit of 0.28 μmol L⁻¹ (S/N=3) was achieved.
  • High precision was observed with a relative standard deviation of 1.0% for 4.0 μmol L⁻¹ ascorbic acid samples.
  • The method showed good agreement with conventional methods when applied to soft drink beverages.

Conclusions:

  • The developed flow injection fluorometric method offers a simple, sensitive, and selective approach for ascorbic acid determination.
  • PBILN serves as an effective fluorescent reagent for this assay.
  • The method is suitable for the practical analysis of ascorbic acid in various beverage samples.