Related Experiment Video
Updated: May 29, 2026
10:43
Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays
Published on: July 20, 2017
Embryonic stem cells and iPS cells: sources and characteristics.
Catherine H Hackett1, Lisa A Fortier
1Department of Clinical Sciences, Box 32, Veterinary Medical Center, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
The Veterinary Clinics of North America. Equine Practice
|August 30, 2011
Summary
Equine regenerative medicine explores pluripotent stem cells for horse health. This review covers characterizing these cells, challenges in horses, and clinical trials using embryonic stem-like (ES) and induced pluripotent stem (iPS) cells.
Area of Science:
- Equine regenerative medicine
- Stem cell biology
- Comparative medicine
Background:
- Regenerative medicine research is rapidly advancing.
- Equine researchers are interested in pluripotent stem cells, which can differentiate into all three primary germ layers.
- Existing literature reports the isolation of equine embryonic stem-like (ES) cells, and other research focuses on generating equine-induced pluripotent stem (iPS) cells.
Purpose of the Study:
- To summarize the essential features required for characterizing pluripotent cells.
- To outline the specific challenges associated with generating pluripotent stem cells in horses.
- To review current and upcoming clinical trials utilizing equine ES and iPS cells.
Main Methods:
- Literature review of equine stem cell research.
- Analysis of criteria for defining pluripotency.
- Compilation of data on challenges in equine stem cell generation.
- Survey of ongoing and future clinical trials involving equine stem cells.
Main Results:
- Pluripotency characterization requires specific markers and functional assays.
- The horse presents unique biological and technical challenges for pluripotent cell generation.
- Several clinical trials are underway or planned, indicating progress in equine regenerative medicine.
Conclusions:
- Establishing robust methods for equine pluripotent stem cell generation is crucial.
- Overcoming species-specific challenges is key to advancing equine regenerative therapies.
- Clinical applications of equine ES and iPS cells hold significant promise for veterinary medicine.
Related Concept Videos
01:06Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic cells are...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
01:13Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:27Source And Potency Of Stem Cells
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...