Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Peptide Bonds02:43

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the bioavailability and bioactivity of thiamine versus allithiamine: studies in <i>Drosophila melanogaster</i> and mice.

Frontiers in nutrition·2026
Same author

Molecular insights into the anti-COVID-19 activity of reduced (quinol) and oxidized (quinone) forms of coenzyme Q10.

Naunyn-Schmiedeberg's archives of pharmacology·2026
Same author

Effectiveness of baloxavir marboxil in nonhuman primates infected with highly pathogenic avian influenza A(H7N9) virus.

EBioMedicine·2026
Same author

Paquinimod Targeting of the S100A8/A9 Axis Suppresses Liver Metastasis in Aged Mice.

Cancers·2026
Same author

Molecular Insights into the Neurogenic Potential of Alpha-lipoic Acid.

Current topics in medicinal chemistry·2026
Same author

Comprehensive analysis of the skeletal phenotype in Chst14-/- mice: implications for dermatan sulfate in bone structure and strength.

Glycobiology·2026

Related Experiment Video

Updated: May 29, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Novel complementary peptides to target molecules.

Hidechika Okada1, Masaki Imai, Fumiko Ono

  • 1Research Institute for Protein Science, Nakayama-cho 2-18, Mizuho-ku, Nagoya 467-0803, Japan. hiokada@med.nagoya-cu.ac.jp

Anticancer Research
|August 30, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a computer program to create complementary peptides (C-peps) that can block inflammatory molecules like C5a. One C-pep, AcPepA, successfully treated sepsis in monkeys by inhibiting C5a-induced inflammation.

More Related Videos

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

Related Experiment Videos

Last Updated: May 29, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid
10:42

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

Area of Science:

  • Biochemistry
  • Immunology
  • Computational Biology

Background:

  • Complement component 5a (C5a) stimulates inflammatory cytokine production, exacerbating conditions like sepsis, particularly in cancer patients.
  • High mobility group box 1 (HMGB1) surge, induced by C5a via the C5L2 receptor, further activates inflammatory cells through Toll-like receptor 4 (TLR4), creating a positive feedback loop.

Purpose of the Study:

  • To develop an evolutionary computer program for generating complementary peptide (C-pep) sequences targeting specific molecules.
  • To evaluate the therapeutic potential of C-peps, specifically AcPepA, in treating C5a-mediated inflammatory conditions such as sepsis.

Main Methods:

  • An evolutionary computer program was utilized to design C-pep sequences by analyzing physico-chemical parameters for interaction with target peptides.
  • Synthesized C-peps were tested for their ability to interfere with target molecule function.
  • The efficacy of a C5a inhibitory C-pep, AcPepA, was assessed in Cynomolgus monkeys challenged with a lethal dose of bacterial lipopolysaccharide (LPS).

Main Results:

  • Approximately 30% of synthesized C-peps demonstrated functional interference with their intended targets.
  • AcPepA effectively rescued Cynomolgus monkeys lethally challenged with LPS when administered intravenously.
  • The therapeutic effect of AcPepA was attributed to the inhibition of C5a-induced HMGB1 surge, thereby mitigating TLR4-mediated inflammatory responses.

Conclusions:

  • The developed evolutionary program successfully generated functional C-peps capable of targeting and inhibiting specific molecules.
  • AcPepA exhibits significant therapeutic potential for treating sepsis by blocking the C5a-mediated inflammatory cascade.
  • Targeting the C5a/C5L2/HMGB1/TLR4 pathway represents a promising strategy for managing sepsis and related inflammatory diseases.