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Related Experiment Videos

Decrease in collagen production in normal and Rous sarcoma virus-transformed chick embryo fibroblasts induced by

K B Delclos, P M Blumberg

    Cancer Research
    |May 1, 1979
    PubMed
    Summary
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    Phorbol 12-myristate 13-acetate (PMA) significantly reduces collagen synthesis in chick embryo fibroblasts. This effect is dose-dependent, reversible, and also observed in Rous sarcoma virus-transformed cells.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Oncology

    Background:

    • Collagen synthesis is a key differentiated function of fibroblasts.
    • Tumor promoters can interfere with normal cellular processes.

    Purpose of the Study:

    • To investigate the impact of phorbol 12-myristate 13-acetate (PMA) on collagen synthesis in chick embryo fibroblasts.
    • To determine if PMA's effect is specific and reversible.

    Main Methods:

    • Measuring [3H]hydroxyproline formation from [3H]proline to quantify collagen synthesis.
    • Utilizing polyacrylamide gel electrophoresis to analyze collagenase-sensitive proteins.
    • Treating normal and Rous sarcoma virus-transformed chick embryo fibroblasts with PMA.

    Main Results:

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    • PMA treatment decreased collagen synthesis in a time-dependent manner, with maximal reduction after 5 days.
    • The decrease in collagen synthesis was specific and not due to effects on prolyl hydroxylase.
    • PMA's inhibitory effect on collagen synthesis was reversible.
    • PMA further reduced collagen synthesis in Rous sarcoma virus-transformed cells.

    Conclusions:

    • PMA is a potent inhibitor of collagen synthesis in chick embryo fibroblasts.
    • The inhibition is not due to cell transformation or selection, as it is reversible.
    • PMA exacerbates the reduced collagen synthesis seen in Rous sarcoma virus-transformed cells.