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Published on: December 13, 2018
[Effect of Mcl-1 antisense oligonucleotide on Hela cell biology and sensitivity of chemotherapy]
Shufang Li1, Jie Zhong, Yongzhong Shi
1Clinic Medical Research Institute, Hunan People's Hospital, Changsha 410005, China. fanger_li@yahoo.com.cn
Objective:
To explore the effect of myeloid leukemia-1 (Mcl-1) gene on the proliferation and apoptosis of Hela cells and the sensitivity of cervical cancer chemotherapy by antisense technology.
Methods:
Mcl-1 antisense oligonucleotide(AS-ODN)was transfected into Hela cells with lipofectamine 2000. The expression of Mcl-1 was analyzed by Western blot, the cell viability was detected by MTT assay, and apoptosis was evaluated by flow cytometry.
Results:
Mcl-1 AS-ODN arrested the cell cycle at G1/S,greatly inhibited the cell growth and induced apoptosis. The sensitivity of Hela cells on chemotherapy was low. There was obvious increase in the apoptosis rate by chemotherapy drugs and growth inhibition rate after inhibiting the expression of Mc1-1.
Conclusion:
Mcl-1 AS-ODN can not only inhibit Hela cell proliferation and induce apoptosis, but also increase the sensitivity of chemotherapy. Mcl-1 may be a potential target gene for cervical cancer chemotherapy.
Insights
Myeloid leukemia-1 (Mcl-1) antisense oligonucleotide inhibits Hela cell growth and induces apoptosis. This approach enhances cervical cancer chemotherapy sensitivity, suggesting Mcl-1 as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Context:
- Cervical cancer remains a significant global health challenge.
- Chemotherapy resistance is a major obstacle in effective cancer treatment.
- Myeloid leukemia-1 (Mcl-1) is an anti-apoptotic protein implicated in cancer progression.
Purpose:
- To investigate the role of Mcl-1 in Hela cervical cancer cells.
- To evaluate the efficacy of Mcl-1 antisense oligonucleotide (AS-ODN) in inhibiting cell proliferation and inducing apoptosis.
- To determine if Mcl-1 inhibition enhances chemotherapy sensitivity in Hela cells.
Summary:
- Mcl-1 AS-ODN was transfected into Hela cells.
- Western blot, MTT assay, and flow cytometry were used to analyze Mcl-1 expression, cell viability, and apoptosis.
- Mcl-1 AS-ODN treatment resulted in G1/S cell cycle arrest, significant growth inhibition, and induced apoptosis.
Impact:
- Mcl-1 AS-ODN effectively inhibits Hela cell proliferation and induces apoptosis.
- Inhibition of Mcl-1 significantly increases the sensitivity of Hela cells to chemotherapy drugs.
- Mcl-1 represents a promising molecular target for overcoming chemotherapy resistance in cervical cancer.
