Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An ancestral transmembrane transcription factor couples cell envelope regulation and the SOS response in Caulobacter crescentus.

PLoS genetics·2026
Same author

Medium-Chain Triacylglycerol Improves Cardiac Function in Chronic Heart Failure: A Randomized Cross-Over Trial.

JACC. Heart failure·2026
Same author

Mitochondrial carrier SLC25A34 links clock, diet, and temperature control of interorganellar lipid cycling.

bioRxiv : the preprint server for biology·2026
Same author

Structural and functional characterization of human kallistatin.

Biochemistry and biophysics reports·2026
Same author

Modulation of surface charges of napin by enzymatic deamidation to improve its techno-functional properties.

Food chemistry·2026
Same author

A framework for building a synthetic cell from the SynCell Asia Initiative.

Nature biotechnology·2026

Related Experiment Video

Updated: May 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative proteomics by amino acid labeling in C. elegans.

Julius Fredens1, Kasper Engholm-Keller, Anders Giessing

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Nature Methods
|August 30, 2011
PubMed
Summary

Researchers labeled Caenorhabditis elegans (C. elegans) using isotope-labeled E. coli. This method identified proteins regulated by nuclear hormone receptor 49, showcasing a powerful tool for C. elegans research.

More Related Videos

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

Related Experiment Videos

Last Updated: May 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

Area of Science:

  • Proteomics
  • Molecular Biology
  • Genetics

Background:

  • Caenorhabditis elegans (C. elegans) is a model organism widely used in biological research.
  • Understanding gene regulation is crucial for deciphering complex biological processes.
  • Nuclear hormone receptors play significant roles in development and physiology.

Purpose of the Study:

  • To develop a method for labeling C. elegans with heavy isotopes for quantitative proteomics.
  • To identify proteins regulated by the nuclear hormone receptor 49 in C. elegans.
  • To demonstrate the utility of combining quantitative proteomics with gene knockdown techniques.

Main Methods:

  • Feeding C. elegans with heavy isotope-labeled Escherichia coli to achieve in vivo labeling.
  • Employing quantitative proteomics to analyze protein expression levels.
  • Utilizing RNA interference (RNAi) for gene knockdown of nuclear hormone receptor 49.

Main Results:

  • Successful labeling of C. elegans with heavy isotope-labeled lysine was achieved.
  • Several proteins were identified as being regulated in response to the loss or knockdown of nuclear hormone receptor 49.
  • The study highlights specific protein targets affected by nuclear hormone receptor 49 activity.

Conclusions:

  • The combined approach of quantitative proteomics and selective gene knockdown is effective for C. elegans research.
  • This methodology provides new insights into the regulatory networks controlled by nuclear hormone receptors.
  • The developed labeling technique offers a valuable tool for future functional genomics studies in C. elegans.