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Updated: May 29, 2026
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged
Fatma-Zohra Tighilt1, Palaniappan Subramanian, Naima Belhaneche-Bensemra
1UDTS, 2, Bd Frantz-Fanon, B.P. 140 Alger-7 merveilles, 16200 Algiers, Algeria.
Abstract:
The paper reports on a novel localized surface plasmon resonance (LSPR) substrate architecture for the immobilization and detection of histidine-tagged peptides. The LSPR interface consists of an ITO (indium tin oxide) substrate coated with gold nanostructures. The latter are obtained by thermal deposition of a thin (2 nm thick) gold film followed by post-annealing at 500 °C. The LSPR interface was coated with poly[3-(pyrrolyl)carboxylic acid] thin films using electrochemical means. The ability of the LSPR interfaces coated with poly[3-(pyrrolyl)carboxylic acid] to chelate copper ions was investigated. Once loaded with metal ions, the modified LSPR interface was able to bind specifically to histidine-tagged peptides. The binding process was followed using LSPR.
