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Updated: May 29, 2026
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Published on: May 17, 2013
NM_001013649.3 gene is down-regulated in human colorectal adenocarcinoma
Qin Jiang1, Chunle Zhang, Yao Chen
1Department of Anatomy, Basic Medical and Forensic Medical Institute, Sichuan University, Chengdu, Sichuan 610041, PR China.
Abstract:
The present study aimed to evaluate the expression of NM_001013649.3 in human colorectal adenocarcinoma tissue and its correlation with clinicopathological aspects of the disease. Through the combination of a cDNA subtractive library and cDNA microarray analysis, a differentially expressed sequence tag ES274081 was identified whose full-length cDNA sequence (NM_001013649.3) was obtained through BLASTn analysis on NCBI. Reverse transcription quantitative real-time PCR was used to assess the expression levels of NM_001013649.3 in cancer and adjacent normal colorectal tissues from 30 patients with colorectal adenocarcinoma. The mRNA levels of NM_001013649.3 were significantly lower in the cancer tissues compared to the adjacent normal colorectal tissues of 25 out of 30 (83%) patients (p<0.005). Furthermore, the rate of down-regulation was significantly higher in moderately differentiated samples (21 out of 23; 91%) compared to poorly differentiated samples (3 out of 6; 50%) (p<0.05). The mRNA levels of NM_001013649.3 are significantly down-regulated in colorectal adenocarcinoma tissue, and this down-regulation is most likely correlated with the differentiation status of the cancer, indicating that NM_001013649.3 is potentially involved in the pathogenesis of colorectal adenocarcinoma.
Insights
The gene NM_001013649.3 shows significantly lower expression in colorectal adenocarcinoma tissues. This down-regulation is linked to the cancer
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Colorectal adenocarcinoma is a significant global health concern.
- Understanding gene expression alterations is crucial for identifying disease mechanisms.
Purpose of the Study:
- To investigate the expression pattern of NM_001013649.3 in colorectal adenocarcinoma.
- To correlate NM_001013649.3 expression with clinicopathological features.
Main Methods:
- Differential gene expression analysis using cDNA subtractive library and microarray.
- Identification of full-length cDNA sequence NM_001013649.3 via BLASTn.
- Quantitative real-time PCR (qRT-PCR) to measure mRNA levels in tumor and adjacent normal tissues.
Main Results:
- NM_001013649.3 mRNA levels were significantly reduced in 83% of colorectal cancer tissues compared to normal adjacent tissues (p<0.005).
- Down-regulation was more pronounced in moderately differentiated tumors (91%) than in poorly differentiated tumors (50%) (p<0.05).
Conclusions:
- NM_001013649.3 is frequently down-regulated in colorectal adenocarcinoma.
- The observed down-regulation correlates with tumor differentiation status.
- NM_001013649.3 may play a role in the pathogenesis of colorectal cancer.
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