Related Experiment Video
Updated: May 29, 2026
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
Published on: June 6, 2025
A multimode optical imaging system for preclinical applications in vivo: technology development, multiscale imaging,
Jae Youn Hwang1, Sebastian Wachsmann-Hogiu, V Krishnan Ramanujan
1Minimally Invasive Surgical Technologies Institute and Department of Surgery, Cedars-Sinai Medical Center, 8700 Beverly Blvd. D6061, Los Angeles, CA 90048, USA. hwangjy@cshs.org
Purpose:
Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging.
Procedures:
We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models.
Results:
The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment.
Conclusions:
This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
