Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Complexometric Titration: Overview

Complexometric Titration: Overview

Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...

Controlled-Current Coulometry: Overview

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...

Diazonium Group Substitution with Halogens and Cyanide: Sandmeyer and Schiemann Reactions

Diazonium Group Substitution with Halogens and Cyanide: Sandmeyer and Schiemann Reactions

Arenediazonium substitution reactions occur when the diazonium group is substituted by various functional groups such as halides, hydroxyl, nitrile, etc. For instance, arenediazonium salts react with copper(I) salts of chloride, bromide, or cyanide to form corresponding aryl chlorides, bromides, and nitriles. These reactions are named Sandmeyer reactions. Although the mechanism of this reaction is complicated, as illustrated in Figure 1, they are believed to progress via an aryl copper...

Gas Chromatography: Overview of Detectors

Gas Chromatography: Overview of Detectors

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Training PBertKla on an Integrated Multi-Source Dataset with a Machine-Learning Layer for Lysine Lactylation Site Prediction.

International journal of molecular sciences·2026

Same author

Soft Ionic Materials: Design and Applications in Functional Electrochemical Systems.

JACS Au·2025

Same author

Discovery of ETI41 and ETI60: novel selective endosomal Toll-like receptor inhibitors for the treatment of autoimmune diseases.

Experimental & molecular medicine·2025

Same author

Blockade of TLR2 activation in macrophages by self-assembled hyaluronic acid nanoparticles alleviates psoriasis-like skin dermatitis.

International journal of biological macromolecules·2025

Same author

A Novel BODIPY-Zn Complex as Innovative Sonosensitizer for Enhanced Sonodynamic Therapy.

Molecules (Basel, Switzerland)·2025

Same author

Rational design and therapeutic potential of MyD88 inhibitory peptide in psoriasis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025

Search research articles

Related Experiment Video

Updated: May 29, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

Coumarin-Cu(II) ensemble-based cyanide sensing chemodosimeter.

Hyo Sung Jung¹, Ji Hye Han, Zee Hwan Kim

¹Department of Chemistry, Korea University, Seoul, 136-704, Korea.

Organic Letters

|August 31, 2011

Summary

A novel chemodosimeter, 1-Cu(II), enables sensitive and selective cyanide detection through a distinct "off-on" fluorescence enhancement. This method shows promise for biological applications, including detection in HepG2 cells.

More Related Videos

Synthesis and Performance Evaluations of ZnCoS/ZnCdS with Twin Crystal Structure for Multifunctional Redox Photocatalysis in Energy Applications

Synthesis and Performance Evaluations of ZnCoS/ZnCdS with Twin Crystal Structure for Multifunctional Redox Photocatalysis in Energy Applications

Published on: July 25, 2025

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

Related Experiment Videos

Last Updated: May 29, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology

Published on: March 12, 2015

Synthesis and Performance Evaluations of ZnCoS/ZnCdS with Twin Crystal Structure for Multifunctional Redox Photocatalysis in Energy Applications

Synthesis and Performance Evaluations of ZnCoS/ZnCdS with Twin Crystal Structure for Multifunctional Redox Photocatalysis in Energy Applications

Published on: July 25, 2025

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

Published on: September 19, 2025

Area of Science:

Analytical Chemistry
Chemical Sensing
Biomedical Applications

Background:

Cyanide is a highly toxic anion requiring sensitive detection methods.
Existing chemodosimeters often lack selectivity or require specific conditions.
Development of fluorescent probes for anion sensing is an active research area.

Purpose of the Study:

To develop an "ensemble"-based chemodosimeter for selective cyanide detection.
To investigate the mechanism of fluorescence change upon cyanide interaction.
To evaluate the potential of the chemodosimeter for biological applications.

Main Methods:

Synthesis and characterization of the chemodosimeter 1-Cu(II).
Spectroscopic studies (fluorescence) to monitor cyanide detection.
Investigation of selectivity against other common anions.
Cellular imaging experiments in HepG2 cells.

Main Results:

The chemodosimeter 1-Cu(II) exhibited a marked "off-on" fluorescence enhancement upon addition of cyanide.
High selectivity for cyanide over other tested anionic species was observed.
The mechanism involves Cu(II) decomplexation and subsequent Schiff base hydrolysis, yielding a fluorescent coumarinaldehyde.
Successful application in selective cyanide detection within HepG2 cells.

Conclusions:

The developed 1-Cu(II) chemodosimeter offers a sensitive and selective platform for cyanide detection.
The fluorescence-based mechanism is well-defined and efficient.
The probe demonstrates potential for real-world biological and environmental monitoring applications.