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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: May 29, 2026

Generation of Induced Regulatory T Cells from Primary Human Na&iuml;ve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Human natural regulatory T cell development, suppressive function, and postthymic maturation in a humanized mouse

Takashi Onoe1, Hannes Kalscheuer, Nichole Danzl

  • 1Department of Surgery, Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 31, 2011
PubMed
Summary

Humanized mice with human fetal thymus/liver and CD34+ cells support normal development and function of CD4(+) regulatory T cells (Tregs). This model enables the study of human Treg biology and therapeutic applications.

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Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
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Published on: July 22, 2011

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Last Updated: May 29, 2026

Generation of Induced Regulatory T Cells from Primary Human Na&iuml;ve and Memory T Cells
14:23

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
14:01

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

Area of Science:

  • Immunology
  • Developmental Biology
  • Transplantation Immunology

Background:

  • CD4(+) regulatory T cells (Tregs) are crucial for immune regulation and self-tolerance.
  • Existing humanized mouse models have limitations in T cell reconstitution and HLA-restricted function.
  • Efficient human immune system reconstitution is essential for studying human immune responses in vivo.

Purpose of the Study:

  • To evaluate the development and function of human Tregs in a novel humanized mouse model.
  • To assess the utility of this model for studying human Treg ontogeny, immunobiology, and therapy.
  • To demonstrate successful multilineage lymphohematopoietic reconstitution including Tregs.

Main Methods:

  • Cotransplantation of human fetal thymus/liver with CD34+ cells into NOD/SCID mice.
  • Analysis of T cell development, including FOXP3(+)Helios(+) natural Tregs, in thymic grafts and peripheral organs.
  • Assessment of Treg phenotypic conversion (CD45 isoform expression) and suppressive function.
  • Evaluation of Ag-specific immune responses and T cell homeostatic expansion.

Main Results:

  • Successful multilineage human lymphohematopoietic reconstitution, including dendritic cells and lymphoid organs.
  • Normal development and presence of FOXP3(+)Helios(+) natural Tregs in thymic grafts and peripheral tissues.
  • Humanized mice exhibited normal Treg phenotypic conversion and suppressive function.
  • Demonstrated Ag-specific immune responses and Treg-dependent T cell expansion.

Conclusions:

  • This humanized mouse model supports normal ontogeny and function of human Tregs.
  • The model provides a valuable platform for investigating human Treg immunobiology and therapeutic strategies.
  • The findings highlight the utility of this model for preclinical studies in immunology and transplantation.