Related Experiment Video
Updated: May 29, 2026

On-Chip Endothelial Inflammatory Phenotyping
Published on: July 21, 2012
Adsorptive modulation of inflammatory mediators dampens endothelial cell activation
Anita Schildberger1, Tanja Buchacher, Viktoria Weber
1Department for Clinical Medicine and Biotechnology, Danube University Krems, Austria.
Aim:
In this study, the effect of specific or selective adsorption of inflammatory mediators on endothelial activation was assessed.
Methods:
Conditioned medium was obtained by stimulation of monocytic THP-1 cells with lipopolysaccharide and treated either with an adsorbent specific for tumour necrosis factor-α or with an albumin-coated polystyrene-divinylbenzene copolymer which selectively binds a range of cytokines. Thereafter, the conditioned medium was applied to endothelial cells in culture.
Results:
Adsorption of inflammatory mediators resulted in significantly decreased endothelial cell activation, as shown by reduced interleukin (IL)-6 and IL-8 secretion from endothelial cells as well as reduced surface expression of the adhesion molecules intercellular adhesion molecule-1 and E-selectin. The effect was more pronounced the earlier the mediator modulation was performed.
Conclusion:
Adsorptive modulation of inflammatory mediators dampens endothelial cell activation and may thus be beneficial as supportive therapy in sepsis.
Related Concept Videos
Inflammatory Response
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Acute Inflammation I: Inflammatory Response
Inflammation
Acute Inflammation III: Local and Systemic Effects
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Inflammatory Response I: Vascular and Cellular
