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Published on: July 8, 2021
Increased matrix metalloproteinase-2 and bone sialoprotein response to human coronal caries
L W Boushell1, H Nagaoka, H Nagaoka
1Department of Operative Dentistry, University of North Carolina, Chapel Hill, NC 27599, USA. boushell@dentistry.unc.edu
Caries Research
|August 31, 2011
Summary
Matrix metalloproteinase-2 (MMP-2) and bone sialoprotein (BSP) accumulate in dentinal tubules affected by early caries, suggesting a role in the host defense mechanism and potential calcification of carious lesions.
Area of Science:
- Biochemistry
- Dental Research
- Immunohistochemistry
Background:
- Host matrix metalloproteinase-2 (MMP-2) in dentin may influence caries progression.
- Bone sialoprotein (BSP) is linked to dentin mineralization and MMP-2 regulation.
Purpose of the Study:
- To determine and contrast the distribution of MMP-2 and BSP in healthy human coronal dentin versus dentin with early caries.
Main Methods:
- Immunohistochemistry was employed on human molars and premolars with and without early caries.
- Monoclonal antibodies against MMP-2 and BSP were used with an avidin-biotin complex method.
- Immunoreactivity was visualized using 3,3'-diaminobenzidine and light microscopy.
Main Results:
- MMP-2 and BSP were absent in the tubule lumens of healthy dentin.
- Intense MMP-2 and BSP immunoreactivity was observed throughout caries-affected dentinal tubules.
- MMP-2 and BSP levels at the dentino-enamel junction remained unchanged.
Conclusions:
- MMP-2 and BSP may be secreted by odontoblasts in response to carious insults.
- Accumulation of MMP-2 and BSP in affected tubules suggests involvement in host defense.
- These proteins might play a role in the calcification process within carious regions.
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