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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 29, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lensless fluorescent microscopy on a chip.

Ahmet F Coskun1, Ting-Wei Su, Ikbal Sencan

  • 1Department of Electrical Engineering, University of California-Los Angeles, CA, USA.

Journal of Visualized Experiments : Jove
|August 31, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a lensless on-chip fluorescent microscope achieving <4 μm resolution over a wide field-of-view. The compact system uses total internal reflection and digital reconstruction for high-throughput applications like cell analysis.

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Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Related Experiment Videos

Last Updated: May 29, 2026

Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics
08:19

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Area of Science:

  • Optics and Photonics
  • Biomedical Engineering
  • Microscopy

Background:

  • Traditional lens-based microscopes are bulky and costly, limiting high-throughput screening.
  • On-chip lensless imaging offers a compact alternative by replacing optical components with advanced theories and algorithms.

Purpose of the Study:

  • To demonstrate a novel on-chip fluorescent microscopy modality without lenses, mechanical scanning, or filters.
  • To achieve high spatial resolution over an ultra-wide field-of-view for biological sample analysis.

Main Methods:

  • Utilizes a prism or hemispherical glass for fluorescent excitation with an incoherent source.
  • Employs total internal reflection (TIR) at the chip's bottom to reject excitation light.
  • Collects fluorescent emission via a fiber-optic faceplate/taper to a CCD sensor.
  • Applies a compressive-sampling based decoding algorithm for image reconstruction.

Main Results:

  • Achieved <4 μm spatial resolution over an ultra-wide field-of-view (>0.6-8 cm²).
  • Successfully imaged vertically stacked micro-channels, enhancing throughput.
  • Demonstrated the potential for rapid processing with a compressive decoder.

Conclusions:

  • The developed lensless on-chip fluorescent microscope is compact and efficient.
  • This platform is valuable for high-throughput cytometry, rare-cell research, and microarray analysis.
  • The technology eliminates the need for bulky optical components, paving the way for simpler, more accessible imaging solutions.