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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: May 29, 2026

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

DNA extraction columns contaminated with murine sequences.

Otto Erlwein1, Mark J Robinson, Simon Dustan

  • 1Jefferiss Research Trust Laboratories, Section of Infectious Diseases, Imperial College London, London, United Kingdom.

Plos One
|August 31, 2011
PubMed
Summary
This summary is machine-generated.

DNA purification columns can yield false positive results for xenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia viruses (pMLVs) in sensitive PCR assays. This contamination impacts research on XMRV in prostate cancer and chronic fatigue syndrome (CFS).

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Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

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Published on: April 4, 2016

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Area of Science:

  • Virology
  • Molecular Biology
  • Oncology

Background:

  • Xenotropic murine leukemia virus-related virus (XMRV) sequences have been identified in human prostate cancer tissues, albeit in low quantities.
  • XMRV and polytropic (p) murine leukemia viruses (MLVs) have also been reported in patients diagnosed with chronic fatigue syndrome (CFS).

Purpose of the Study:

  • To assess the prevalence of XMRV in human prostate cancer tissue samples.
  • To investigate potential sources of contamination in highly sensitive molecular detection methods.

Main Methods:

  • Polymerase chain reaction (PCR) assays were employed to detect specific viral DNA sequences.
  • Primers were designed to identify genomic mouse DNA and XMRV.
  • Analysis included eluates from DNA purification columns and various DNA samples.

Main Results:

  • Amplification products, initially thought to be mouse DNA contamination, were detected in DNA purification column eluates.
  • Subsequent PCR analysis revealed sequences of XMRV and pMLVs in these eluates, as well as in mouse DNA, human DNA, and DNA of unspecified origin.
  • These findings indicate that DNA purification columns can be a source of contamination.

Conclusions:

  • DNA purification columns can introduce contaminants that interfere with the detection of minute DNA targets using sensitive amplification techniques like PCR.
  • This contamination poses a significant challenge for studies investigating the presence of viruses such as XMRV in clinical samples, including prostate cancer and CFS research.
  • Careful consideration of reagent and column contamination is crucial for accurate molecular diagnostics and research.