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Heat-labile phosphatase simplifies the preparation of dephosphorylated vector DNA
1Epicentre Technologies, Madison, WI 53713.
Gene
|March 30, 1990
Abstract:
A novel enzyme for DNA dephosphorylation, HK phosphatase, is completely and irreversibly inactivated at 65 degrees C. HK phosphatase treatment of BamHI-digested pBR322 reduces nonrecombinants in cloning experiments to approx. 5% of transformant colonies.