Related Experiment Video
Updated: May 29, 2026

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Development of an amperometric biosensor for phenol detection
L M C Silva1, A M Salgado, M A Z Coelho
1Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro (UFRJ), E-122, University City, CEP 21949-909, Rio de Janeiro, Brazil.
Abstract:
With the aim of searching for an in situ method for monitoring phenol, Agaricus bisporus tissue with tyrosine activity was used as a biocomponent and an oxygen electrode used as a transducer to develop a biosensor. The experimental methodology investigated the relation between dissolved oxygen and phenol concentration using a standard solution. Biosensor calibration was evaluated by studying reaction time and tissue amount necessary to promote a reliable response and to minimize errors. The influence of air saturation of the sample and washing of the electrode was also investigated. Results showed that 5 g of mushroom tissue with a 1 min reaction time promoted the best biosensor response within a phenol concentration range of 5-10 ppm. Washing of the electrode did not change the performance of the analysis; however, initial air saturation caused less variation amongst the samples.
Related Concept Videos
Amperometry: Overview
Microbial Biosensors
Potentiometry: Membrane Electrodes
