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Related Experiment Video

Updated: May 29, 2026

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

Published on: November 17, 2014

Pattern recognition receptor gene expression in ischemia-induced flap revascularization.

Thomas R Howdieshell¹, Lauren McGuire, Joann Maestas

¹Department of Surgery, University of New Mexico HSC, Albuquerque, NM, USA. thowdieshell@salud.unm.edu

|September 1, 2011

Summary

Early expression of pattern recognition receptors (PRRs) in ischemic wounds drives inflammation and new blood vessel growth. This innate immune response is crucial for healing and flap revascularization after injury.

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Last Updated: May 29, 2026

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

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Published on: November 17, 2014

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

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In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

Published on: June 8, 2013

Area of Science:

Immunology
Regenerative Medicine
Wound Healing

Background:

The innate immune system initiates inflammation in response to infection or tissue damage.
Pattern recognition receptors (PRRs) detect microbial and endogenous danger signals.
Understanding PRR dynamics in wound healing is critical.

Purpose of the Study:

To investigate the temporal relationship between PRR gene expression, wound perfusion, and flap revascularization.
To analyze gene expression patterns in ischemic wound tissue.

Main Methods:

Surgical creation of myocutaneous flaps in mice.
Laser speckle contrast imaging to assess flap ischemia and revascularization.
Microarray analysis and qRT-PCR to quantify gene expression of PRRs and related factors.

Main Results:

Laser speckle imaging accurately predicted ischemia and revascularization patterns.
Histology showed leukocyte infiltration and new blood vessel growth (angiogenesis).
Significant early gene expression of PRRs, cytokines, and growth factors was observed, peaking at 48 hours.

Conclusions:

Surgical and ischemic injury triggers early PRR gene expression.
PRRs may bind endogenous molecules, initiating inflammatory and angiogenic gene transcription.
This response is key to wound inflammation and angiogenesis in flap survival.