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Published on: May 8, 2020
CoREST/LSD1 control the development of pyramidal cortical neurons
Patricio Fuentes1, José Cánovas, F Andrés Berndt
1Program in Physiology and Biophysics, Institute for Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Independencia 1027, Santiago, Chile.
Cerebral Cortex (New York, N.Y. : 1991)
|September 1, 2011
Summary
Depleting CoREST delays neuron development and migration in the mouse cerebral cortex. This epigenetic regulator impacts cell cycle and radial migration, independent of NRSF/REST.
Area of Science:
- Neuroscience
- Developmental Biology
- Epigenetics
Background:
- Neuronal development involves complex stages from proliferation to differentiation.
- The orchestration of these developmental processes remains largely unelucidated.
- Chromatin remodeling complexes play a role in regulating gene expression during development.
Purpose of the Study:
- To investigate the role of CoREST in the development of cortical pyramidal neurons.
- To understand how CoREST influences neuronal precursor cell dynamics and migration.
- To explore the epigenetic mechanisms governing neural development in the cerebral cortex.
Main Methods:
- In utero gene expression manipulation in mouse embryonic cerebral cortex.
- Depletion of CoREST (Co-Repressor of Elements in Transcription) to assess its function.
- Analysis of neuronal precursor cell populations, cell cycle, morphology, and migration dynamics.
Main Results:
- CoREST depletion significantly delays the transition between multipolar and bipolar stages in newborn cortical pyramidal neurons.
- Loss of CoREST function impairs the onset of radial migration.
- CoREST deficiency transiently increases progenitor cell populations but does not affect glutamatergic fate acquisition.
Conclusions:
- CoREST is crucial for the timely execution of neural development programs, particularly radial migration.
- CoREST's function in this context is independent of RE-1 Silencing Transcription Factor (REST) and requires LSD1.
- Epigenetic regulation by CoREST is vital for proper cerebral cortex development.
