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Updated: May 29, 2026

High-throughput Detection Method for Influenza Virus
10:05

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

Evaluation of real-time reverse transcriptase PCR assays for detection of pandemic influenza A/H1N1 2009 virus

G R Chidlow, G B Harnett, D J Speers

    Journal of Clinical Microbiology
    |September 1, 2011
    PubMed
    Abstract

    No abstract available in PubMed .

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    Real Time RT-PCR

    Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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