Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitochondrial binding sites for triiodothyronine.

R L Greif, D Sloane

    Endocrinology
    |November 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Thyroid hormone (T3) distribution in rat liver mitochondria was studied. Rapid T3 uptake occurred in nuclei and mitochondria, suggesting specific binding may not be essential for rapid metabolic effects.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Active metabolite concentration of clopidogrel in patients taking different doses of aspirin: results of the interaction trial.

    Journal of thrombosis and haemostasis : JTH·2015
    Same author

    Randomized controlled trial of a new portable calf compression device (Venowave) for prevention of venous thrombosis in high-risk neurosurgical patients.

    Journal of thrombosis and haemostasis : JTH·2011
    Same author

    Desensitization regimens for drug allergy: state of the art in the 21st century.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2011
    Same author

    Agglutination of Endamoeba histolytica cysts.

    The American journal of tropical medicine and hygiene·2010
    Same author

    Oral allergy syndrome.

    Allergy and asthma proceedings·2001
    Same author

    Advances in hospital outcomes research.

    Journal of health services research & policy·1998
    Same journal

    Zingerone ameliorates ovarian impairment by regulating steroidogenesis and apoptosis in letrozole-treated mouse.

    Endocrinology·2026
    Same journal

    Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

    Endocrinology·2026
    Same journal

    Long Non-Coding RNAs Mediate Endocrine Signaling and Resistance in Prostate Cancer.

    Endocrinology·2026
    Same journal

    Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

    Endocrinology·2026
    Same journal

    Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

    Endocrinology·2026
    Same journal

    Discovery proteomics identification of factors contributing to gonadotropin β expression.

    Endocrinology·2026
    See all related articles

    Area of Science:

    • Endocrinology
    • Cell Biology
    • Biochemistry

    Background:

    • Thyroid hormones, like thyroxine (T4) and triiodothyronine (T3), regulate metabolism.
    • Rapid metabolic effects of thyroid hormones on liver mitochondria are known.
    • The subcellular localization and binding characteristics of T3 are not fully understood.

    Purpose of the Study:

    • To investigate the subcellular distribution of [125I]L-T3 in rat liver mitochondria and nuclei.
    • To determine the effect of a T3 loading dose on hormone distribution.
    • To explore the potential role of specific mitochondrial binding sites in rapid thyroid hormone action.

    Main Methods:

    • Intraperitoneal injection of high specific activity [125I]L-T3 in rats.
    • Subcellular fractionation to isolate liver mitochondria and nuclei.

    Related Experiment Videos

  • Chromatographic analysis to confirm the identity of the radiolabeled hormone.
  • Assessment of [125I]L-T3 distribution with and without a T3 loading dose in normal and thyroidectomized rats.

    • Main Results:

    • Within 2 minutes, [125I]L-T3 was found in both nuclear and mitochondrial fractions.
    • A T3 loading dose decreased nuclear T3 and increased mitochondrial T3 in normal rats.
    • Hypothyroid rats showed higher nuclear and mitochondrial T3 levels compared to normal rats.
    • No significant effect of stable T3 on mitochondrial membrane-bound [125I]L-T3 was observed.

    Conclusions:

    • Specific, high-affinity mitochondrial binding sites for T3 were not evident 2 minutes post-injection.
    • Nuclear binding sites for T3 appear to be established rapidly.
    • Specific binding may not be a prerequisite for the rapid metabolic effects of thyroid hormones on liver mitochondria.