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Updated: May 29, 2026
11:08
A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
K-acetylation and its enzymes: overview and new developments.
Juliette Adjo Aka1, Go-Woon Kim, Xiang-Jiao Yang
1The Rosalind & Morris Goodman Cancer Research Center, McGill University, Montréal, QC, Canada.
Handbook of Experimental Pharmacology
|September 1, 2011
Summary
Lysine acetylation, a reversible posttranslational modification, is dynamically regulated by enzymes. Understanding lysine acetylomes and their associated enzymes (KATs and KDACs) is crucial for therapeutic development.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Lysine acetylation is a reversible posttranslational modification involving the transfer of an acetyl group from acetyl-CoA to lysine residues.
- This process is regulated by lysine acetyltransferases (KATs) and lysine deacetylases (KDACs), historically known as histone deacetylases (HDACs) in eukaryotes.
- Recent proteomic studies reveal extensive lysine acetylation across bacterial and eukaryotic proteomes, comparable in scale to phosphoproteomes.
Purpose of the Study:
- To provide an overview of lysine acetylomes, KATs, KDACs, and AcK-recognizing domains.
- To highlight recent advancements in understanding acetylation and its modulators.
- To emphasize the therapeutic potential of targeting acetylation pathways.
Main Methods:
- Literature review and synthesis of current research findings.
- Analysis of proteomic data identifying acetylated proteins.
- Discussion of enzyme families involved in acetylation regulation.
Main Results:
- Hundreds of bacterial and thousands of eukaryotic proteins are acetylated.
- Lysine acetylation plays a significant role in cellular signaling and pathophysiology.
- AcK-recognizing domains are critical for the function of acetylated proteins.
Conclusions:
- Further research is needed to assign specific enzymes to acetylation events and understand their impact.
- Targeting KATs and KDACs offers potential for novel therapeutic strategies.
- The book chapters will delve deeper into HDAC families for therapeutic insights.
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