Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Channel Rhodopsins01:11

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Lewy body pathology and phenotypic associations in patients with mild cognitive impairment: Evidence from the Interceptor study.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

Inter-assay and Inter-site Performance of Alpha-Synuclein Seed Amplification Assays in Synucleinopathies: A Multicenter 2 × 2 Protocol Comparison.

Neurology and therapy·2026
Same author

Variants in the proteasome regulator PSMF1 cause a phenotypic spectrum from parkinsonism to perinatal lethality.

Nature communications·2026
Same author

Clinical correlates of a negative cerebrospinal fluid α-synuclein seed amplification assay result in Parkinson's disease.

NPJ Parkinson's disease·2026
Same author

Prevalence and clinical effects of Lewy Body pathology in non-prion rapidly progressive dementias: a retrospective cohort study.

Alzheimer's research & therapy·2026
Same author

Plasma and CSF proteomic signatures related to Alzheimer's, α-synuclein, or vascular pathologies and clinical decline.

medRxiv : the preprint server for health sciences·2026
Same journal

Predicting Nirmatrelvir Resistance in SARS-CoV-2 M<sup>pro</sup> Mutants with an Integrated Computational Framework.

The journal of physical chemistry. B·2026
Same journal

From Cation Solvation to Anion Coordination: Lewis-Acidic Boranes Enable Halide Salt Electrolytes.

The journal of physical chemistry. B·2026
Same journal

In Vitro-Prepared A30P Alpha-Synuclein Fibrils Adopt the Conserved and Disease-Relevant Greek Key Fold.

The journal of physical chemistry. B·2026
Same journal

Metastructure Analysis of Self-Assembled Nanocubes with Different Equatorial Methyl Groups Based on Molecular Dynamics Simulations.

The journal of physical chemistry. B·2026
Same journal

A Cocoordinated <sup>1</sup>H Internal Reference Quantifies Proton-Exchange Bias in Coordinated-Water Diffusion.

The journal of physical chemistry. B·2026
Same journal

Unveiling Electrolyte-Dependent Coordination Site Dynamics for Redox Mediator Design in Lithium-O<sub>2</sub> Batteries: Exchange vs Rearrangement.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Building a Simple and Versatile Illumination System for Optogenetic Experiments
06:41

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

A chiroptical photoswitchable DNA complex.

Angela Mammana1, Gregory T Carroll, Jetsuda Areephong

  • 1Centre for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, Groningen 9747 AG, The Netherlands.

The Journal of Physical Chemistry. B
|September 2, 2011
PubMed
Summary
This summary is machine-generated.

This study shows that dithienylethene (DET) molecular switches bind to DNA via electrostatic interactions. This binding influences both DNA and switch structures, allowing for chiroptical property modulation through photochemical reactions.

Related Experiment Videos

Last Updated: May 29, 2026

Building a Simple and Versatile Illumination System for Optogenetic Experiments
06:41

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

Area of Science:

  • Nanoscale smart materials
  • Supramolecular chemistry
  • Molecular electronics

Background:

  • DNA's unique properties offer potential for advanced materials.
  • Molecular switches can be integrated with biomolecules for novel functions.

Purpose of the Study:

  • To investigate the binding of amine-terminated dithienylethene (DET) molecular switches to synthetic DNA.
  • To explore the structural and chiroptical consequences of this DNA-switch complexation.
  • To understand the driving forces and pH-dependence of the binding interaction.

Main Methods:

  • UV-vis spectroscopy to detect spectral changes upon binding.
  • Circular dichroism (CD) spectroscopy to assess chiroptical properties.
  • Photochemical reactions to switch DET isomers.
  • pH-dependent binding studies.

Main Results:

  • DET molecular switches bind to double-stranded DNA through electrostatic interactions.
  • DNA induces chirality in the DET switch, and the switch alters DNA structure, evidenced by CD and UV-vis spectra.
  • Photochemical switching of DET isomers modulates the complex's chiroptical properties.
  • Binding is pH-dependent, with protonated amines and DNA phosphates being key interaction sites.

Conclusions:

  • Amine-terminated DET switches form supramolecular complexes with DNA.
  • This complexation enables the modulation of chiroptical properties via photochemical control.
  • Electrostatic interactions are crucial for the binding, highlighting potential for DNA-based molecular devices.