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Related Concept Videos

Inflammation01:38

Inflammation

Overview
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
GPCR Desensitization01:12

GPCR Desensitization

G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
Acute Inflammation I: Inflammatory Response01:26

Acute Inflammation I: Inflammatory Response

Acute inflammation is a rapid, short-lived physiological response to tissue injury or infection, designed to eliminate harmful agents and initiate repair. This tightly regulated process typically lasts from minutes to several days and is triggered by factors such as microbial invasion, physical trauma, or chemical injury.Recognition and Mediator ReleaseThe inflammatory response begins when resident immune cells—such as mast cells, macrophages, and dendritic cells—detect damage-associated...

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Related Experiment Video

Updated: May 29, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

SNARE complex-mediated degranulation in mast cells.

Joseph R Woska1, Marc E Gillespie

  • 1Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, St. John's University, Jamaica, NY, USA. joseph.woska@boehringer-ingelheim.com

Journal of Cellular and Molecular Medicine
|September 2, 2011
PubMed
Summary
This summary is machine-generated.

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are key to mast cell degranulation in allergic and autoimmune diseases. Targeting specific SNAREs offers a novel therapeutic strategy for inflammatory conditions.

More Related Videos

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Related Experiment Videos

Last Updated: May 29, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Mast cell degranulation is crucial in allergic and autoimmune diseases.
  • Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediate vesicle fusion and cargo release in mast cells.
  • The precise roles of SNARE complexes in mast cell degranulation require further elucidation.

Purpose of the Study:

  • To review the expression, formation, and localization of SNARE proteins in mast cells.
  • To identify key SNARE family members involved in mast cell degranulation.
  • To discuss RNA interference (RNAi) and small interfering RNA (siRNA) as therapeutic strategies.

Main Methods:

  • Review of recent and historical data on SNARE proteins in murine and human mast cells.
  • Summarization of functional data implicating specific SNAREs in mast cell degranulation.
  • Discussion of RNA interference (RNAi) and siRNA for validating SNARE function and therapeutic potential.

Main Results:

  • SNARE proteins and their complexes are integral to mast cell secretory vesicle transport and fusion.
  • Specific SNAREs have been identified as critical players in mast cell degranulation.
  • RNAi and siRNA present viable methods for targeting SNAREs to modulate mast cell function.

Conclusions:

  • SNARE proteins represent novel therapeutic targets for allergic and autoimmune diseases.
  • Understanding SNARE complex function in mast cells is essential for developing new treatments.
  • Targeting SNAREs via siRNA offers a promising approach for managing inflammatory diseases.