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Updated: May 29, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Nitrification and potential control mechanisms in simulated premises plumbing
Mohammad Shahedur Rahman1, Gem Encarnacion, Anne K Camper
1Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.
Nitrification in drinking water systems is affected by copper and chloramine, with copper surfaces showing faster inhibition by chloramine. Nitrification recovers after inhibitor removal, but recovery rates vary by treatment and surface type.
Area of Science:
- Environmental microbiology
- Water chemistry
- Wastewater treatment
Background:
- Nitrification is crucial for ammonia removal in drinking water.
- Household plumbing materials can influence microbial processes like nitrification.
- Understanding nitrification resilience is vital for maintaining water quality.
Purpose of the Study:
- To investigate the impact of copper and polyvinyl chloride (PVC) surfaces on nitrification.
- To assess the effects of chemical inhibitors (copper, chlorite, chloramine) on established nitrification.
- To evaluate the recovery of nitrification after inhibitor exposure.
Main Methods:
- Established nitrification in glass reactors with copper or PVC surfaces using indigenous microorganisms.
- Simulated household plumbing conditions with stagnant and flowing water cycles.
- Introduced various nitrification inhibitors and monitored ammonia and nitrate levels.
- Measured copper release from copper surfaces during inhibitor testing.
Main Results:
- Copper presence did not significantly inhibit nitrification.
- Chlorite showed limited effect on PVC but inhibited nitrification in copper reactors at 20 ppm.
- Chloramine at a 5:1 ratio significantly impacted and stopped nitrification, with faster inhibition in copper systems.
- Copper release increased when inhibitors were tested.
- Nitrification recovered after inhibitor removal, with varying recovery rates.
Conclusions:
- Copper surfaces are more susceptible to chloramine inhibition than PVC.
- Nitrification resilience is influenced by plumbing materials and chemical stressors.
- Copper release is a concern during nitrification inhibition events.
- Further research is needed to understand long-term effects and optimize water treatment strategies.
Related Concept Videos
Microbes and the Nitrogen Cycle
Bioreactor Controls-I
Microbial Wastewater Treatment
Biological Treatment of Effluent and Waste Water
Environmental Applications of Microorganisms
Inorganic Nitrogen Assimilation

