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Updated: May 29, 2026

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

Profiling of the BRCA1 transcriptome through microarray and ChIP-chip analysis.

Julia J Gorski¹, Kienan I Savage, Jude M Mulligan

¹Centre for Cancer Research and Cell Biology, Queen's University Belfast, Belfast BT9 7BL and ALMAC Diagnostics, Craigavon BT63 5QD, UK. j.gorski@qub.ac.uk

Nucleic Acids Research

|September 2, 2011

Summary

This summary is machine-generated.

The BRCA1 protein influences gene transcription genome-wide, but directly binds only a few gene promoters. BRCA1 acts as part of a complex that responds to DNA damage, revealing its role in transcriptional regulation.

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Published on: February 28, 2021

Area of Science:

Molecular Biology
Genetics
Cancer Research

Background:

The role of BRCA1 in regulating gene transcription is known, but its genome-wide impact remains unclear.
Understanding BRCA1's transcriptional regulatory network is crucial for cancer biology.

Purpose of the Study:

To comprehensively define the genome-wide transcriptional regulation by BRCA1.
To investigate the direct and indirect mechanisms of BRCA1's transcriptional control.

Main Methods:

Genome-wide expression profiling (microarray analysis).
Chromatin immunoprecipitation followed by chip analysis (ChIP-chip).
Co-immunoprecipitation assays to identify protein interactions.

Main Results:

BRCA1 depletion altered 1294 genes, but BRCA1 directly bound only 44 promoters.
27% of BRCA1-bound transcripts were involved in transcriptional regulation, explaining indirect effects.
BRCA1 binds to promoters of genes not affected by its loss but regulated upon DNA damage.
BRCA1 interacts with transcription factors like AP2-α, PAX2, and ZF5.

Conclusions:

BRCA1's transcriptional regulation is largely indirect, mediated through interactions with various transcription factors.
BRCA1 functions as part of a poised complex at specific promoters, ready to respond to genotoxic stress.
These findings elucidate BRCA1's multifaceted role in maintaining genomic stability and transcriptional homeostasis.