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Related Experiment Video

Updated: May 29, 2026

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
07:38

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

Single-particle virus tracking.

Michael J Rust, Melike Lakadamyali, Boerries Brandenburg

    Cold Spring Harbor Protocols
    |September 2, 2011
    PubMed
    Summary
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    This study details a protocol for tracking individual virus particles during cell infection using live-cell imaging and single-particle tracking. Fluorescent markers enable precise observation of viral entry and infection dynamics in real-time.

    Area of Science:

    • Virology
    • Cell Biology
    • Biophysics

    Background:

    • Understanding viral infection mechanisms at the single-particle level is crucial for developing antiviral therapies.
    • Live-cell imaging and single-particle tracking (SPT) offer powerful tools to visualize dynamic biological processes.
    • Previous methods lacked the resolution or adaptability to track individual virus particles effectively during infection.

    Purpose of the Study:

    • To provide a detailed protocol for performing single-particle virus-tracking experiments.
    • To enable researchers to observe and analyze individual virus particle behavior during cellular infection.
    • To facilitate the study of viral entry pathways and infection kinetics.

    Main Methods:

    • Utilizing real-time, live-cell imaging techniques.

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    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

    Published on: May 26, 2013

    Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
    14:59

    Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

    Published on: September 7, 2009

    Related Experiment Videos

    Last Updated: May 29, 2026

    Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper
    07:38

    Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

    Published on: April 9, 2017

    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
    08:31

    Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

    Published on: May 26, 2013

    Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
    14:59

    Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

    Published on: September 7, 2009

  • Employing single-particle tracking (SPT) algorithms to follow viral trajectories.
  • Incorporating one or more fluorescent markers for enhanced visualization of virus particles.

    • Main Results:

    • The protocol allows for the successful tracking of individual virus particles within live cells.
    • Real-time data provides insights into the dynamic interactions between viruses and host cells.
    • Fluorescent labeling ensures high signal-to-noise ratio for accurate trajectory analysis.

    Conclusions:

    • This protocol offers a robust method for studying virus-cell interactions at the single-particle level.
    • The technique can be adapted for various viruses and cell types.
    • It provides a foundation for investigating viral pathogenesis and designing novel interventions.