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Related Experiment Video

Updated: May 29, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Single-cell electroporation in Xenopus.

Xue Feng Liu, Kurt Haas

    Cold Spring Harbor Protocols
    |September 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Single-cell electroporation (SCE) precisely delivers molecules to individual neurons in vivo. This method enables detailed study of neuron growth and function using advanced imaging techniques.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biotechnology

    Background:

    • Single-cell electroporation (SCE) is a key method for introducing charged molecules into cells.
    • Targeting individual cells within complex tissues remains a challenge.

    Purpose of the Study:

    • To describe a method for in vivo-targeted single-cell electroporation (SCE) of individual tectal neurons in Xenopus laevis tadpoles.
    • To highlight the utility of SCE combined with in vivo two-photon microscopy for studying neuron development and function.

    Main Methods:

    • Developed a focal electroporation technique using a pipette electrode with a tip diameter smaller than the target cell.
    • Localized the electric field to restrict pore formation to the single cell in contact with the electrode tip.
    • Combined single-cell electroporation with in vivo two-photon microscopy for 3D imaging.

    More Related Videos

    Electroporation of Craniofacial Mesenchyme
    07:23

    Electroporation of Craniofacial Mesenchyme

    Published on: November 28, 2011

    In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
    10:49

    In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

    Published on: February 15, 2017

    Related Experiment Videos

    Last Updated: May 29, 2026

    Single Cell Electroporation in vivo within the Intact Developing Brain
    13:31

    Single Cell Electroporation in vivo within the Intact Developing Brain

    Published on: July 11, 2008

    Electroporation of Craniofacial Mesenchyme
    07:23

    Electroporation of Craniofacial Mesenchyme

    Published on: November 28, 2011

    In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
    10:49

    In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

    Published on: February 15, 2017

    Main Results:

    • Demonstrated precise delivery of macromolecules to individual tectal neurons in vivo.
    • Showcased the ability to visualize neuron growth and cell-autonomous effects of altered protein function in 3D.
    • Discussed factors influencing SCE efficiency and its diverse applications.

    Conclusions:

    • Focal SCE provides a highly specific method for molecular delivery to individual neurons within intact tissues.
    • The combination of SCE and in vivo imaging opens new avenues for understanding neuronal development and function at the single-cell level.