Related Experiment Video
Updated: May 29, 2026
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Nitrite exhaled breath condensate study in patients undergoing cardiopulmonary bypass cardiac surgery
Viviane Dos Santos Augusto1, Graziela Saraiva Reis, Verena Kise Capellini
1Programa de Aprimoramento em Fisioterapia Divisão de Cirurgia Cardiotorácica, Hospital das Clínicas de Ribeirão Preto, FMRP, USP.
Background:
There is a relative lack of studies on postoperative changes in nitrite (NO2 - ) concentrations, a marker of injury, following cardiac surgery. In this context, investigations on how exhaled NO concentrations vary in the postoperative period of cardiac surgery will certainly contribute to new clinical findings.
Objective:
The objective of this study was to compare the EBC NO levels in both the pre and postoperative (24 hours) periods of cardiac surgery.
Methods:
Twenty - eight individuals were divided into three groups: 1) control, 2) coronary artery bypass grafting, and 3) valve surgery. The nitrite (NO2 - ) levels were measured by chemiluminescence in blood samples and exhaled breath condensate (EBC). Data were analyzed by the Mann - Whitney and Wilcoxon tests.
Results:
1) Preoperatively, the EBC NO2 - levels from groups 2 and 3 patients were higher than control individuals; 2) The postoperative (24 hours) NO2 - levels in the EBC from group 3 patients were lower compared with preoperative values; 3) The NO2 - levels in the plasma from group 2 patients were lower in the preoperative compared with the postoperative (24h) values and; 4) Preoperatively, there was no difference between groups 2 and 3 in terms of plasma NO2 - concentrations.
Conclusion:
These data suggest that NO measurement in EBC is feasible in cardiac surgery patients.
