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Published on: May 23, 2025
New urea biosensor based on urease enzyme obtained from Helycobacter pylori
Bahar Dindar1, Emine Karakuş, Fatih Abasıyanık
1Department of Chemistry, Faculty of Science and Arts, Yildiz Technical University, Esenler, Istanbul, Turkey.
Applied Biochemistry and Biotechnology
|September 2, 2011
Summary
Researchers developed a novel urea biosensor using Helicobacter pylori urease immobilized on a PVC ammonium electrode. This sensitive and stable biosensor allows for rapid urea determination in human serum.
Area of Science:
- Biotechnology
- Biosensor Development
- Enzyme Immobilization
Background:
- Helicobacter pylori urease is crucial for its survival and pathogenicity.
- Urea biosensors are valuable tools for clinical diagnostics and environmental monitoring.
- Developing stable and sensitive biosensors requires optimized enzyme immobilization and electrode materials.
Purpose of the Study:
- To develop and characterize a new urea biosensor utilizing urease from Helicobacter pylori.
- To optimize the performance of the biosensor by investigating key parameters like pH, temperature, and buffer concentration.
- To compare the analytical performance of the H. pylori-based biosensor with one derived from Jack bean urease.
Main Methods:
- Isolation of urease enzyme from Helicobacter pylori.
- Immobilization of urease onto a poly(vinylchloride) (PVC) ammonium membrane electrode using nonactine.
- Optimization of pH, buffer concentration, temperature, urease concentration, stirring rate, and immobilization techniques.
- Electrochemical characterization and performance evaluation of the urea biosensor.
- Comparison with a urea biosensor prepared using Jack bean urease.
Main Results:
- The optimized biosensor demonstrated a linear working range of 1 × 10(-5) to 1 × 10(-2) M urea.
- Optimal conditions were found at pH 6.0, 5 mM buffer concentration, and 25 °C.
- The biosensor exhibited high sensitivity, stability for over 2 months, and a rapid response time of 1-2 minutes.
- Both H. pylori and Jack bean urease-based biosensors enabled rapid determination of urea in human serum.
Conclusions:
- A novel and effective urea biosensor was successfully developed using immobilized Helicobacter pylori urease.
- The biosensor offers excellent analytical parameters, including high sensitivity and stability, making it suitable for practical applications.
- This technology facilitates rapid and accurate urea quantification in biological samples, with potential clinical utility.
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