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Related Experiment Videos

What's new in the lymphocyte phenotype?

D B Jones1

  • 1University Department of Pathology, Southampton General Hospital, England.

Pathology, Research and Practice
|April 1, 1990
PubMed
Summary

The development of monoclonal antibodies and standardized nomenclature has revolutionized lymphocyte subset identification. These advancements provide crucial tools for cell phenotyping and understanding immune cell development.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Early methods for identifying lymphocyte subsets, such as surface immunoglobulin detection and cell rosetting, are now considered rudimentary.
  • The advent of monoclonal antibodies has dramatically improved the characterization of normal and abnormal lymphocyte populations.

Purpose of the Study:

  • To highlight the evolution of lymphocyte subset identification techniques.
  • To emphasize the impact of monoclonal antibodies and standardized nomenclature on cell phenotyping.

Main Methods:

  • Development of hybridoma technology for continuous antibody-producing cell lines.
  • Standardization of monoclonal antibody nomenclature through international workshops.
  • Application of diverse techniques for cell staining and phenotyping.

Main Results:

  • A vast array of monoclonal antibodies is now available for cell phenotyping.
  • International workshops have standardized nomenclature, with 45 "Clusters of Differentiation" (CD) identified by 1986.
  • These studies have yielded significant functional and molecular biological insights.

Conclusions:

  • Monoclonal antibodies and standardized nomenclature (CD) represent a major leap in immunology.
  • These tools are essential for sophisticated analysis of lymphocyte subsets in health and disease.

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