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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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Related Experiment Video

Updated: May 29, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

High dynamic range bio-molecular ion microscopy with the Timepix detector.

Julia H Jungmann1, Luke MacAleese, Jan Visser

  • 1FOM Institute for Atomic and Molecular Physics (AMOLF), Science Park 104, 1098 XG Amsterdam, The Netherlands.

Analytical Chemistry
|September 3, 2011
PubMed
Summary

A new Timepix detector with microchannel plates enables detailed imaging of large biomolecules using time-of-flight mass spectrometry. This technology provides both mass spectra and localization data for peptides and proteins up to 78 kDa.

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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Instrumentation Science

Background:

  • Time-of-flight mass spectrometry imaging (TOF-MSI) is crucial for analyzing biomolecules.
  • Existing detectors face limitations in dynamic range and resolution for large biomolecules.

Purpose of the Study:

  • To develop and evaluate a novel detector system for enhanced TOF-MSI of large biomolecules.
  • To demonstrate the capability of capturing mass-resolved ion images with both spectral and spatial information.

Main Methods:

  • Utilized a 512 × 512 pixel Timepix detector coupled with microchannel plates (MCPs).
  • Performed matrix-assisted laser desorption ionization (MALDI) on a commercial ion microscope.
  • Captured time-resolved images of various m/z species in a single measurement.

Main Results:

  • The MCP-Timepix assembly achieved a significantly increased dynamic range.
  • Obtained mass spectra and localization data for peptide and protein standards, including ubiquitin oligomers up to 78 kDa.
  • Demonstrated prolonged MCP lifetime and optimized gain settings for improved image quality.

Conclusions:

  • The developed MCP-Timepix system offers advanced capabilities for TOF-MSI of large biomolecules.
  • This technology provides a powerful tool for mass-spectral and spatial analysis in biological samples.
  • The system's performance indicates potential for broader applications in proteomics and molecular imaging.