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Examination of latent infection in germfree mice for some murine viruses
Abstract:
Sera from some groups of germfree mice were examined for reactivity against Sendai, reo 3, Theiler's GD VII, ectromelia, and mouse hepatitis virus antigens. Of 51 sera collected in September and October 1976 and in August and September 1977 from one germfree mouse breeding colony, there was no positive reaction against all the antigens tested. Moreover, the attempt of virus isolation from homogenates of various organs including lungs, liver, kidneys, and intestines of 6 germfree mice was unsuccessful.
Insights
Germfree mice sera showed no reactivity against common viral antigens. Virus isolation attempts from organs of these mice were also unsuccessful, indicating a lack of exposure to these specific viruses.
Area of Science:
- Immunology
- Virology
- Microbiology
Background:
- Germfree mice are raised in sterile environments, lacking exposure to microorganisms.
- Assessing immune responses and viral presence in germfree models is crucial for understanding disease dynamics.
Purpose of the Study:
- To evaluate the serological reactivity of germfree mouse sera against specific viral antigens.
- To determine the presence of infectious viruses within germfree mice through organ isolation attempts.
Main Methods:
- Sera from germfree mice were tested for antibody responses against Sendai, reo 3, Theiler's GD VII, ectromelia, and mouse hepatitis virus.
- Virus isolation was attempted from homogenates of lungs, liver, kidneys, and intestines of germfree mice.
Main Results:
- No positive serological reactions were observed against any of the tested viral antigens in the germfree mouse sera.
- No viruses were successfully isolated from the organs of the examined germfree mice.
Conclusions:
- The germfree mice studied did not exhibit evidence of exposure to the specific viruses tested.
- These findings support the sterile status of the germfree mouse colony concerning the investigated viral agents.