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Related Experiment Videos

Sample, count, identify, and store the mutans streptococci.

I del Rio Gomez1, D Bratthall

  • 1Department of Cariology, School of Dentistry, University of Lund, Malmö, Sweden.

Scandinavian Journal of Dental Research
|April 1, 1990
PubMed
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The "Strip mutans" method allows viable storage of mutans streptococci for over a year when frozen. Stored at room temperature, viability decreased to approximately 8 weeks, impacting further bacterial analysis.

Area of Science:

  • Microbiology
  • Dental Research
  • Bacteriology

Background:

  • Mutans streptococci are key pathogens in dental caries.
  • Accurate sampling methods are crucial for studying these bacteria.
  • The "Strip mutans" method offers a novel approach to sample and store these bacteria.

Purpose of the Study:

  • To determine the viability of mutans streptococci stored on "Strip mutans" under various conditions.
  • To assess the feasibility of long-term storage for subsequent bacterial analyses.
  • To identify the serotype/biotype of mutans streptococci from international samples.

Main Methods:

  • A novel "Strip mutans" method involving saliva contamination of a plastic strip, incubation, drying, and storage.
  • Viability testing of bacteria stored under frozen, frozen in glycerine, and room temperature dried conditions.

Related Experiment Videos

  • Biochemical and immunological analyses of colonies from international samples.

    • Main Results:

    • Mutans streptococci remained viable for at least 1 year when "Strip mutans" were stored frozen or frozen in glycerine.
    • Viability decreased to approximately 8 weeks when strips were stored dried at room temperature.
    • Serotype/biotype c/e/f of Streptococcus mutans were most frequent in international samples; Streptococcus sobrinus (d/g) was also identified.

    Conclusions:

    • The "Strip mutans" method provides a viable storage solution for mutans streptococci, especially when frozen.
    • Frozen storage ensures bacterial viability for at least one year, facilitating extensive downstream analyses.
    • The method successfully identified common mutans streptococci types from diverse geographical locations.