Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

What is a Species?

What is a Species?

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluating the statistical utility and information loss in the transformation of a real-world oncology database from CDISC-SDTM to OMOP-CDM.

ESMO real world data and digital oncology·2026

Same author

A phylogenomic and morphometric reassessment of Ovulidae highlights host-related shell shape evolution.

Molecular phylogenetics and evolution·2026

Same author

Cognitive behavioural therapies with a trauma focus for children and adolescents: a commentary on a systematic review and meta-analysis.

Journal of family & child health·2026

Same author

HPV circulating tumor DNA to monitor response to pembrolizumab and vorinostat combination in patients with advanced HPV-related squamous-cell carcinomas.

ESMO open·2025

Same author

Fatigue and management of warfighter mental endurance.

BMJ military health·2025

Same author

Development and validation of AI-assisted transcriptomic signatures to personalize adjuvant chemotherapy in patients with pancreatic ductal adenocarcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Search research articles

Related Experiment Video

Updated: May 29, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

ABGD, Automatic Barcode Gap Discovery for primary species delimitation.

N Puillandre¹, A Lambert, S Brouillet

¹UMR 7138, Muséum National d'Histoire Naturelle, Departement Systématique et Evolution, Paris, France.

Molecular Ecology

|September 3, 2011

Summary

Automatic Barcode Gap Discovery (ABGD) is a fast, efficient method for assigning organisms into species using DNA barcodes. It identifies species by analyzing genetic divergence, aiding in taxonomic classification.

More Related Videos

Application of DNA Barcoding to Identify Medicinal Plants

Application of DNA Barcoding to Identify Medicinal Plants

Published on: November 1, 2024

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

Related Experiment Videos

Last Updated: May 29, 2026

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles

Published on: July 11, 2025

Application of DNA Barcoding to Identify Medicinal Plants

Application of DNA Barcoding to Identify Medicinal Plants

Published on: November 1, 2024

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

Area of Science:

Genomics
Bioinformatics
Taxonomy

Background:

DNA barcoding is crucial for species identification in uncharacterized groups.
Identifying species boundaries requires analyzing genetic divergence within and between populations.

Purpose of the Study:

To develop an automated method for species delimitation using DNA barcode data.
To establish a robust procedure for partitioning sequence data into putative species based on genetic divergence.

Main Methods:

The Automatic Barcode Gap Discovery (ABGD) method was developed.
ABGD utilizes the barcode gap principle: intraspecific divergence is less than interspecific divergence.
A model-based confidence limit for intraspecific divergence is inferred and used to detect the barcode gap for recursive partitioning.

Main Results:

ABGD demonstrated computational efficiency across six metazoan datasets.
The method performed well with standard intraspecific divergence values, except in cases of low sampling.
Simulations revealed sensitivity to recent speciation events and large species numbers.

Conclusions:

ABGD provides a fast and simple approach for preliminary species delineation from sequence data.
The method is a valuable tool for integrative taxonomy, requiring complementary evidence for validation.