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A novel test tube method of screening for hemoglobin E
1Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. asittt@chiangmai.ac.th
International Journal of Laboratory Hematology
|September 3, 2011
Summary
A new, simple test tube method effectively screens for Hemoglobin E (HbE), a common blood disorder. This cost-effective approach aids in preventing HbE/β-thalassemia, especially in resource-limited settings.
Area of Science:
- Hematology
- Medical Diagnostics
- Biochemistry
Background:
- Hemoglobin E (HbE) is a prevalent β-structural variant globally.
- HbE can lead to severe hereditary hemolytic anemia (HbE/β-thalassemia) when combined with β-thalassemia.
- Effective screening for HbE is crucial for managing HbE/β-thalassemia.
Purpose of the Study:
- To introduce a novel, accessible test tube method for screening Hemoglobin E (HbE).
- To provide an alternative to existing screening methods for HbE, particularly in resource-limited areas.
Main Methods:
- A diethyl aminoethyl (DEAE)-cellulose resin-based test tube assay was developed.
- HbE/A(2) remains in the supernatant, indicated by a red color, while other Hbs bind to the resin.
Main Results:
- The method demonstrates accuracy and efficiency comparable to standard cellulose acetate electrophoresis.
- It is a low-cost, simple technique requiring no specialized equipment.
- Reagents are stable for up to 5 months, and samples remain suitable for testing for the same duration.
Conclusions:
- This novel test tube method offers a viable alternative for mass population screening of HbE.
- It is particularly suitable for small healthcare facilities with limited resources.
