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Updated: May 29, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method
S Pornprasert1, S Panyasai, J Waneesorn
1Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand. sakornmi001@gmail.com
Introduction:
Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS).
Methods:
The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups.
Results:
Mean ± SD of Hb CS level of the homozygote was not significantly different from that of the Hb H-CS disease (1.9 ± 1.8 vs. 2.8 ± 1.3, P = 0.13), but it was significantly higher than that of the heterozygote (1.9 ± 1.8 vs. 0.4 ± 0.2, P = 0.007). The MCV <70 fL was found in Hb H-CS disease only.
Conclusion:
CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70 fL), this approach provided a high resolution to identify Hb H-CS disease.
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