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Related Experiment Video

Updated: May 29, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

The 'alternative' EMT switch.

Pamela Klingbeil¹, Clare M Isacke

¹Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. pamela.klingbeil@icr.ac.uk

Breast Cancer Research : BCR

|September 3, 2011

Summary

Alternative RNA splicing drives epithelial to mesenchymal transition (EMT) by switching CD44 expression to the standard isoform. This switch is crucial for EMT and linked to high-grade breast cancer and apoptosis resistance.

Area of Science:

Molecular Biology
Cancer Research
Developmental Biology

Background:

Epithelial to mesenchymal transition (EMT) is vital for embryonic development but also implicated in disease progression.
Alternative RNA splicing is increasingly recognized as a regulator of cellular processes.
The role of CD44 standard isoform expression in EMT and cancer requires further elucidation.

Purpose of the Study:

To investigate the role of alternative RNA splicing in EMT.
To determine the specific contribution of CD44 isoforms to EMT.
To link CD44 standard isoform expression to breast cancer progression and therapeutic resistance.

Main Methods:

Analysis of alternative RNA splicing events during EMT.
Characterization of CD44 isoform expression.

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Investigation of CD44 standard isoform function in epithelial cells.

Assessment of CD44 standard isoform in a mouse model of breast cancer.

Main Results:

A switch from CD44 variant isoforms to the standard isoform is necessary for EMT.
CD44 standard isoform expression is required for normal epithelial cells to undergo EMT.
CD44 standard isoform expression correlates with high-grade breast cancer.
CD44 standard isoform is linked to phosphoinositide 3-kinase/Akt pathway activation and apoptosis resistance in a mouse model.

Conclusions:

Alternative RNA splicing, specifically the switch to CD44 standard isoform, is a critical regulator of EMT.
CD44 standard isoform expression is a potential biomarker for aggressive breast cancer.
Targeting the CD44 standard isoform may offer new therapeutic strategies for breast cancer.