Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars
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Updated: May 29, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Xiaodong Tao1, Oscar Azucena, Min Fu
1Jack Baskin School of Engineering, University of California, Santa Cruz, Santa Cruz, California 95064, USA. taoxd@soe.ucsc.edu
Abstract:
We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and 3× improvement in the signal intensity for fixed mouse tissues at depths of 70 μm.
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