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Updated: May 29, 2026

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
Published on: September 6, 2012
Guest-release control in enzyme-sensitive, amphiphilic-dendrimer-based nanoparticles through photochemical
Krishna R Raghupathi1, Malar A Azagarsamy, S Thayumanavan
1Department of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Abstract:
Stimuli sensitive, facially amphiphilic dendrimers have been synthesized and their enzyme-responsive nature has been determined with dual fluorescence responses of both covalently conjugated and non-covalently bound reporter units. These dual responses are correlated to ascertain the effect of enzymatic action on micellar aggregates and the consequential guest release. The release of the guest molecule is conveniently tuned by stabilizing the micellar aggregates through photochemical crosslinking of hydrophobic coumarin units. This photo-crosslinking is also utilized as a tool to investigate the mode of enzyme-substrate interaction in the context of aggregate-monomer equilibrium.
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