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Updated: May 29, 2026
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
Fibronectin and bone morphogenetic protein-2-decorated poly(OEGMA-r-HEMA) brushes promote osseointegration of
Xiaoshuai Ren1, Yuanzi Wu, Yan Cheng
1Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, 100871 Beijing, PR China.
Abstract:
To be better used as medical implants in orthopedic and dental clinical applications, titanium and titanium-based alloys need to be capable of inducing osteogenesis. Here we describe a method that allows the facile decoration of titanium surfaces to impart an osteogenesis capacity. A Ti surface was first deposited on a poly(OEGMA-r-HEMA) film using surface-initiated atom-transfer radical polymerization (SI-ATRP) with the further step of carboxylation. The modified surfaces were resistant to cell adhesion. Fibronectin (FN) and recombinant human bone morphogenetic protein-2 (rhBMP-2) were further immobilized onto p(OEGMA-r-HEMA) matrices. Our results demonstrate that the FN- and rhBMP-2-conjugated polymer surfaces could induce the adhesion of MC3T3 cells on Ti surfaces. Moreover, the protein-tethered surface exhibited enhanced cell differentiation in terms of alkaline phosphatase activity compared to that of the pristine Ti surface at similar cell proliferation rates. This research establishes a simple modification method of Ti surfaces via Ti-thiolate self-assembled monolayers (SAMs) and SI-ATRP and identifies a dual-functional Ti surface that combines antifouling and osseointegration promotion.
