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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
A cholera outbreak associated with drinking contaminated well water
Reza Ranjbar1, Mohammad Rahbar, Ali Naghoni
1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Archives of Iranian Medicine
|September 6, 2011
Summary
A 2008 cholera outbreak in Qazvin, Iran, was caused by a single clone of Vibrio cholerae serotype Inaba. Investigations revealed contaminated well water as the outbreak
Area of Science:
- Epidemiology
- Microbiology
- Public Health
Background:
- Cholera outbreaks pose significant public health challenges.
- An acute diarrheal illness outbreak occurred in Qazvin, Iran, in 2008.
- The outbreak affected participants of a wedding ceremony.
Purpose of the Study:
- To identify the causative agent of the outbreak.
- To determine the source and extent of the outbreak.
- To investigate an acute diarrheal illness outbreak in Qazvin, Iran.
Main Methods:
- Epidemiological, environmental, and microbiological investigations were conducted.
- Clinical and environmental samples were analyzed for Vibrio cholera.
- Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) was used to determine strain relationships.
Main Results:
- The outbreak's attack rate was 21.8%.
- Vibrio cholerae serotype Inaba was identified in clinical and environmental samples.
- A single clone of V. cholerae, confirmed by ERIC-PCR, caused the outbreak.
Conclusions:
- The investigation identified well water as the source of the cholera outbreak.
- A single clone of V. cholerae serotype Inaba was responsible.
- Contaminated well water led to the 2008 Qazvin cholera outbreak.
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