Replication of herpes simplex virus in human B lymphocytes stimulated by Epstein-Barr virus

Human B lymphocytes prepared from adult blood or cord blood by the use of neuraminidase-treated sheep red blood cells which did not respond mitogenically to phytohemagglutinin or pokeweed mitogen, responded well to the B95-8 strain of Epstein-Barr virus, but not to the P3HR-1 strain, with increased incorporation of tritiated thymidine. Replication of herpes simplex virus could be demonstrated in cultures of B lymphocytes preincubated with the B95-8 strain.