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Poly(adenosine diphosphate ribose) synthesis during herpes simplex virus infection.
Intervirology
|January 1, 1979
Summary
Herpes simplex virus (HSV) infection immediately halts host cell DNA synthesis while initiating viral DNA replication. Poly(adenosine diphosphate ribose) polymerase activity remained unchanged during these processes.
Area of Science:
- Virology
- Molecular Biology
- Cellular Biology
Background:
- Herpes simplex virus (HSV) is a common human pathogen.
- Viral infections can significantly disrupt host cell functions, including DNA synthesis.
- Understanding these disruptions is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the immediate effects of HSV infection on host cellular DNA synthesis.
- To examine the onset and extent of viral DNA synthesis post-infection.
- To determine the role of poly(adenosine diphosphate ribose) polymerase activity in these cellular changes.
Main Methods:
- Infection of baby hamster kidney cells with HSV.
- Measurement of cellular DNA synthesis rates.
- Quantification of HSV DNA synthesis.
- Assay of poly(adenosine diphosphate ribose) polymerase activity.
Main Results:
- HSV infection rapidly inhibited cellular DNA synthesis in host cells.
- Extensive HSV DNA synthesis was observed immediately following infection.
- Poly(adenosine diphosphate ribose) polymerase activity showed no significant alteration during the observed period.
Conclusions:
- HSV exerts immediate and profound control over host DNA synthesis machinery.
- Viral DNA replication commences rapidly after infection.
- The observed alterations in DNA synthesis are independent of poly(adenosine diphosphate ribose) polymerase activity in the early stages of infection.