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Glycerol as a reference material for fecal fat quantitation using low-resolution time domain ¹H NMR spectroscopy
James P Corsetti1, Judy Sterry, Manisha Sakpal
1Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA. James_Corsetti@urmc.rochester.edu
Objectives:
To assess glycerol as reference material for low-resolution time-domain (1)H NMR analysis of fecal fat.
Design And Methods:
NMR analysis of fecal fat in stool samples with added glycerol was used to assess linearity, recovery, and relationship with NMR lipid signal.
Results:
The study revealed for added glycerol excellent linearity (r=0.9998), recovery (101-104%), and linear relationship with simulated fecal fat content.
Conclusions:
Glycerol is an effective reference material for NMR fecal fat analysis.
